Nyotalia

Nyotalia refers to the genderbent versions of the canon Hetalia characters, it's a fan-made term started by the Japanese fandom. Nyo deprives from the words nyotaika (male-female genderbents) and Talia is a short reference for Hetalia or Italia. The characters who get genderbent are usually called Nyo!, Fem! (male-female) or Male! (female-male).

In addition, designs of the opposite gender exist for certain characters, as Hidekaz Himaruya drew them for fun to see what the nations would be like. However, Himaruya also noted that when drawing the characters, he came up with their designs as if they were completely different people (if they were designed as females to begin with). This is presumably why some of them lack certain distinguishing details that their original versions had. However, their personalities mostly remain the same.

Female North Italy

 * Voiced by Rena Maeda in Japanese.

Female North Italy mostly inherits her male counterpart version in terms of appearances; she has a curlier and lighter brown hair that is tied into a ponytail with her strange curl sticking out from the left side of her head, she has the same brown eyes that appears to be more amber and is opened more times than her canon version. If look closely, there's a bandage on her left knee. She usually wears a short khaki shirt with a skirt of the same colour, a black tie, a belt, a pair of brown gloves and a pair of golden brown leather boots.

Himaruya described Fem! North Italy as strong, unlike her "useless" male counterpart as she can display an unusual bout of physical strength when angered. She had also argued and throw punches with Female England, to an extent that she is the braver one and more willing to fight. As shown in her bandage on her left knee, she's more unaware of accident proneness and is rougher than her canon version.

Female Germany

 * Voiced by Rika Zaiki in Japanese.

Female Germany does not wear her blonde hair slicked back. In a group sketch, she is shown to wear only a tank top and pants when training, much like the male Germany. Two other sketches depict her wearing uniforms similar to her male counterpart's. Each illustration depicts her with a worried or exasperated expression.

In his notes, Himaruya described her as being reserved, serious like her male counterpart, and as having large breasts. He also said that she's tall and gives off a cool feeling. In a sketch, Germany was shown to be scolding a bruised male Italy for rashly getting himself into difficult situations.

Female Japan
Female Japan is depicted as to be the same height as his male version and has a bob hairstyle, and has shine to her eyes unlike her male counterpart, with them being more amber in the coloured version. In Himaruya's notes, she is stated to be a Yamato Nadeshiko-type character who yearns for the West a bit.
 * Voiced by Mai Goto in Japanese.

She is usually not sketched in any sort of military uniform, but rather in a simple pink and purple kimono or other civilian clothes such as a school uniform. She seems to always have a shy expression, befitting of one with a Yamato personality. According to Himaruya, Japan used to have much longer hair in the past. She cut it to its current length after opening up her borders in order to prove she would be able to get along with the rest of the world.

Female America

 * Voiced by Ryoko Shintani in Japanese.

The female version of America does not have "Texas" glasses or a "Nantucket" hair. She has blue eyes and wavy, honeysuckle-coloured hair that comes to her shoulders, with two star-shaped barrettes holding her bangs out of her face

She often dresses in short skirts or shorts and wears tops which expose her midriff. In her initial design, she also wore a cowboy hat and boots and had an American flag patch on her top, though some fans mistook it for a tattoo on her chest. Her later design depicts her in a bomber jacket like the male America, though she seems to be fond of hot dogs instead of hamburgers. Her weapon of choice appears to be a baseball bat as she solves her problems through violence.

Female England

 * Voiced by Yuuko Kurose in Japanese.

England, unlike canon England, has glasses. She no longer has the signature thick eyebrows, though Himaruya mentioned missing them, and wears her blonde hair in long pigtails that are either slightly curled or completely straight, not in a messy hairstyle such as her male version. In two coloured pictures of her, her hair appears to be the same colour as her male counterpart's original hair colour. Her eyes are the same colour of green.

This version of the character is called Igiko (girl England/UK) by Himaruya and Japanese fans alike. Himaruya describes her as trying to behave elegantly, but being quite punk. In an old post, Himaruya commented that being kuudere and that being the type to say embarrassing things smoothly might be nice for a female England. She is often depicted in a British schoolgirl uniform or the uniform of the female nurses of the NHS.